用流动注射法研究巯基化合物-铈(Ⅳ)-氢化可的松的化学发光反应

Study on Chemiluminescence Reaction of Mercapto Compound-Cerium (Ⅳ)-Hydrocortisone by Flow Injection Analysis

采用流动注射法研究了巯基化合物－铈（Ⅳ）－氢化可的松（ＨＣＲｓ）体系的化学发光行 为，对影响化学发光强度的诸因素进行试验和探讨，建立了流动注射化学发光法检测谷胱甘 肽（ＧＳＨ），半胱氨酸（Ｃｙｓ）等含巯基化合物的新方法。检测ＧＳＨ和Ｃｙｓ和线性范围分别为 ２．０×１０－６～１０．０×１０－５ｍｏｌ／Ｌ和１．０×１０－６ｍｏｌ／Ｌ～１０．０×１０－５ｍｏｌ／Ｌ；检测限分别为２．０ ×１０－７ｍｏｌ／Ｌ和１．４×１０－６ｍｏｌ／Ｌ（Ｓ／Ｎ＝３）；ＧＳＨ、Ｃｙｓ加入血清中进行回收测定的回收率 为９０％～９５％，相对标准偏差小于４％。

A new chemiluminescence (CL) reaction of -SH-Ce (Ⅳ )-HCRs was studied by flow-injection method in 1. 0 mol/L H2SO4. The factors influencing CL intensity were investigated. The new method based on CL reaction for the determination of glutathione (GSH) and cysteine (Cys ) has been established by optimizing the experimental conditions. The method allows the determination in the ranges of 2. 0 × 10-6 - 10. 0 × 10-5 mol/L for GSH and 1. 0 × 10-6- 10. 0 × 10-5 mol/L for Cys with detection limits of 2. 0 × 10-7 mol/L and 1. 4 × 10-6 mol/L (S/N= 3) for GSH and Cys respectively. The recoverics of GSH and Cys are between 90% and 95% from human serum.