摘要
采用流动注射法研究了巯基化合物-铈(Ⅳ)-氢化可的松(HCRs)体系的化学发光行 为,对影响化学发光强度的诸因素进行试验和探讨,建立了流动注射化学发光法检测谷胱甘 肽(GSH),半胱氨酸(Cys)等含巯基化合物的新方法。检测GSH和Cys和线性范围分别为 2.0×10-6~10.0×10-5mol/L和1.0×10-6mol/L~10.0×10-5mol/L;检测限分别为2.0 ×10-7mol/L和1.4×10-6mol/L(S/N=3);GSH、Cys加入血清中进行回收测定的回收率 为90%~95%,相对标准偏差小于4%。
A new chemiluminescence (CL) reaction of -SH-Ce (Ⅳ )-HCRs was studied by flow-injection method in 1. 0 mol/L H2SO4. The factors influencing CL intensity were investigated. The new method based on CL reaction for the determination of glutathione (GSH) and cysteine (Cys ) has been established by optimizing the experimental conditions. The method allows the determination in the ranges of 2. 0 × 10-6 - 10. 0 × 10-5 mol/L for GSH and 1. 0 × 10-6- 10. 0 × 10-5 mol/L for Cys with detection limits of 2. 0 × 10-7 mol/L and 1. 4 × 10-6 mol/L (S/N= 3) for GSH and Cys respectively. The recoverics of GSH and Cys are between 90% and 95% from human serum.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
1997年第6期679-682,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目
关键词
化学发光
巯基化合物
铈
氢化可的松
GSH
CYS
Flow-injection, chemiluminescence, mercapto compound, cerium (Ⅳ), hydrocortisone