现场扫描隧道显微镜研究有机相中的蛋白质结构

Study on the Structure of Hemoglobin in Organic Solvents by In situ STM

本文用现场扫描隧道显微镜(STM)研究了血红蛋白(Hb)分子在二类不同性质的有机溶剂中的结构变化.结果表明:在疏水性有机溶剂CC14中Hb分子结构几乎与其在水溶液中的结构一样,保持天然的结构;而在亲水性有机溶剂DMF中,组成Hb分子的两个二聚体分离,肽链部分舒展,表明其二、三级结构和四级结构遭到不同程度的破坏.

In situ STM has been used to study the structure of hemoglobin (Hb) in two kinds of organic media. In hydrophobic organic solvent such as carbon tetrachloride, the structure of Hb is almost the same as in aqueous solution, similar to its native structure. However, when in hydrophilic organic solvent such as dimethylformamide, the two dimers of Hb molecule become separate and unfold to a certain extent.