多羟基化合物化学发光分析新体系的研究——尿液中尿酸的测定

New Chemiluminescence Reaction System for Analysis of Polyhydroxy-compounds——Determination of Uric Acid in Urine

本文首次提出使用luminol-K7[Cu(IO_6)_2]-KOH化学发光体系,结合流动注射技术进行多羟基化合物的测定.方法基于多羟基化合物能增强luminol与K7[Cu(IO_6)2]反应产生的化学发光,且浓度与增强作用的大小呈线性关系.利用该法进行尿酸的测定,发现尿酸在2×10^-8～1×10^(-6)g/mL浓度范围内与化学发光强度呈线性关系,检出限为7.2×10^-9 g/mL,相对标准偏差为2.04%.

A novel flow injection chemiluminescence method for the determination of polyhydroxy -compounds was described. The method is based on that polyhydroxy - compounds enhance the CL reaction of luminol with K7[Cu(IO6)2] in potassium hydroxide medium. In the experiments,luminescent reagent--luminol was injected in order to keep the baseline steady and savereagents,especially,to improve sensitivity. The method was used in measuring uric acid in urineand it allows quantitation of uric acid concentration in the 2×10-8 × 10-6g/mL range with adetection limit of 7.2 × 10-9g/mL. RSD=2.04 % .