母亲和新生儿GSTM1、GSTT1和细胞色素P_(450)1A1基因多态性与早产易感性关系

Polymorphisms of GSTM1,GSTT1 and CYP1A1 Genes in mothers and neonates related to susceptibility to preterm delivery

目的探讨母亲和新生儿GSTM1、GSTT1基因以及细胞色素P4501A1基因多态性对早产的影响。方法采用病例-对照调查方法,应用多重PCR和限制性内切酶PCR(RFLP-PCR)技术对早产母亲和对照母亲以及早产新生儿和对照新生儿GSTM1、GSTT1基因和CYP1A1基因MSPI位点多态性分别进行检测。结果GSTM1、GSTT1基因和CYP1A1基因MSPI位点多态性在早产母亲和对照母亲组中没有显著性差异(P>0.05);以上基因在早产新生儿和对照新生儿组中也没有显著性差异(P>0.05)。GSTM1与GSTT1联合基因型在母亲和新生儿的病例与对照组中也没有显著性差异(P>0.05);GSTT1缺失型和CYP1A1变异型联合基因在早产母亲组与对照母亲组中有显著性差异(χ2=4.683,P<0.05,OR=2.440)。结论携带GSTT1缺失型基因以及CYP1A1变异型基因的母亲,其发生早产的危险性增大。

Objective Objective Based on the distribution of genetic polymorphisms of GSTM1, GSTT1 and CYP1A1 genotypes in mothers and neonates with preterm delivery and health controls, to explore the relationship between these gene polymorphisms and susceptibility to preterm delivery. Methods we investigated mothers and neonates with preterm delivery and health controls were investigated by case-control method. The frequencies of GSTM1, GSTT1 and CYPIA1 genotypes were detected by PCR and RFLP-PCR techniques. Results The difference of variation frequencies of GSTM1, GSTT1 and CYPIA1 （Msp I polymorphism） genotypes between cases （mothers and neonates respectively）and their health controls were not statistically significant（ P 〉 0.05）. The difference of variation frequencies of GSTM1 and GSTI＇I combined between mother cases group and control groups were not statistically significant（ P 〉 0.05）. The neonates was same as mother groups. However, the differences of CYPIA1 heterozygous mutation and homozygous mutation gene combined with the null genotypes among GSTT1 between mother groups with preterm delivery and health controls were statistically significant （ X2 = 4.683, P 〈 0.05, OR = 2.440）. Conclusion CYPIA1 heterozygous mutation and homozygous mutation gene combined with GSTT1 null genotypes in mothers could increase the risk of preterm delivery.