高效液相色谱法测定蔬菜中类胡萝卜素组分

Several carotenoids of vegetable determinated by high performance liquid chromatography

目的测定食物中叶黄素（Lut）、隐黄素（Cryp）、α-胡萝卜素（a-C）、β-胡萝卜素（β-C）和番茄红素（Lyco）的水平。方法采用高效液相色谱法（HPLC）。HPLC色谱柱：Develosil C30柱；流动相：A（甲醇：叔丁基甲基醚：水=83：15：2），B（甲醇：叔丁基甲基醚：水：8：90：2），梯度洗脱；检测器：紫外-可见光检测器；检测波长：450nm。结果Lut在0、013～0．508,ug／ml范围内与峰面积的线性相关，R。=0、999，加标回收率为105．9％，RSD=4、62％；Cryp在0．015～0、605μg／m1范围内与峰面积的线性相关，R^2=1．000，加标回收率为94．7％，RSD=3．50％；n—C在0．011～0．432μg／ml范围内与峰面积的线性相关，R^2=1．000，加标回收率为95．8％，RSD：3．83％；、β-C在0、013～0、518μg/ml范围内与峰面积的线性相关，R^2=1．000，加标回收率为108．1％，RSD=2、97％；Lyco在0．012～0．498μg／ml范围内与峰面积的线性相关，R^2：1、000，加标回收率为104．5％，RSD：4．24％。结论该方法同时测定了蔬菜中的Lut、Cryp、α—C、β-C和Lyco5种类胡萝卜素组分，方法简便、快速、准确。

Objective To detect a-carotene （α-C）, β-carotene （β-C）, cryptoxanthin （Cryp）, lutein （Lut） and lycopene （Lyco） levels in vegetables. Methods A HPLC method with C30 column was used to detect several carotenoids at the wave length of 450nm. A gradient elution method with methanol, methyl-t-butyl-ether and ammonium acetate solution was used to separate the carotenoids. Results The level of Lut, Cryp, α-c, β-c and lycopene were linearly related with the peak area. The recoveries of the standard were between 94.7%-108.1%. The relative standard deviations were less than 4.62% .Conclusion The method could be simple and credible. It could separate 5 carotenoids in vegetables simultaneously.