新疆维吾尔族妇女宫颈癌组织HPV16型E6基因变异分析

Analysis of mutations in the E6 oncogene of human papillomavirus 16 in Xinjiang Uygur women with cervical carcinoma

目的:新疆维吾尔族妇女宫颈癌的发生主要与人乳头状瘤病毒16(HPV16)感染相关,特定的HPV16E6突变株具有更高的致癌危险性。本研究通过检测维吾尔族妇女宫颈癌组织中HPV16E6基因突变情况,探讨其与维吾尔族妇女宫颈癌高发的关系。方法:从140例维吾尔族妇女宫颈癌石蜡包埋组织中提取DNA作为模板,PCR扩增HPV16E6全长基因,PCR产物直接测序,进行突变分析。结果:本组维吾尔族妇女宫颈癌组织中HPV16的阳性率是73.6%(103/140),91例E6基因测序及分析结果表明,维吾尔族妇女宫颈癌组织中存在HPV16E6变异株,其中49例(53.8%)分离株发生L83V突变,4例(4.39%)发生D25E突变,2例(2.20%)发生D64E/L83V突变,36例(39.6%)与野生型相同。结论:新疆维吾尔族妇女宫颈癌患者HPV16E6基因发生变异,主要以L83V突变为主,HPV16变异株在维吾尔族妇女宫颈癌患者中的分布可能与维吾尔族妇女宫颈癌高发存在一定关系。

Objective：High-risk human papillomaviruses（ HRHPVs）, especially HPV16, is the major cause of cervical carcinoma（CC） in Xinjing Uygur women. In some region, specific E6 mutation is considered as dangerous factor causing CC. This study was designed to investigate distribution of HPV16 mutation and the relationship between the mutation and high incidence of CC in Uygur women. Methods ：The tissue DNA was extracted from 140 CC paraffin embedded tissue of Uygur women. HPV16 E6 gene was amplified by polymerase chain reaction（PCR） from the CC tissue DNA. The PCR fragments were sequenced and analyzed. Results：The result of PCR showed that the positive rate of HPV16 was 73.5% （103/140） ; Ninty-one of 97 PCR fragments were sequenced and analyzed. Thirty-six of them maintained prototype（39. 6% ）. Forty-nine have L83V mutation（53.6% ） and 4 have D25E（4. 25% ）, and 2 have L83V/D64E（2. 27% ）. Conclusion：There are mutations within the HPV16 E6 gene in CC of Xinjiang Uygur women. The most common mutation was L83V. Our research suggested that the distribution of HPV16 E6 mutation might be associated with high incidence of CC in Uygur.