esp1在A549和Hela细胞中的表达及对染色体倍性的影响

esp1 expression and its effect on ploidy in A549 and Hela cells

目的观察esp1(estradiol-stimulated protein)在A549和Hela两种肿瘤细胞株中的表达与染色体倍性的关系。方法提取肿瘤细胞A549、Hela及分别转染pIRE-S2-EGFP-ESP1的相应肿瘤细胞总RNA,逆转录,real-time quantitative PCR检测,比较esp1在肿瘤细胞中的表达变化;VIDEOTEST-KARYO3.1分析相应的染色体数目变化;流式细胞仪检测其染色体倍性的改变。结果转染pIRE-S2-EGFP-ESP1后esp1表达上调(P<0.01),染色体数目减少(P<0.01),染色体倍性降低(P<0.01)。结论esp1上调表达可以抑制肿瘤细胞异常分裂相,降低染色体倍性,但是esp1在A549和Hela两种肿瘤细胞株中的表达没有观察到明显差异。

Objective To observe the relation of estradiol-stimulated protein （esp1） expression with chromosome ploidy in A549 and Hela cells. Methods Total RNA was extracted from A549 and Hela cells respectively for cDNA. pIRE-S2-EGFP-ESP1 plasmid was transfected into the 2 corresponding tumor cells. Real-time quantitative PCR was used to detect esp1 gene expression. The chromosome changes were analyzed by VIDEOTEST-KARYO 3.1 and chromosome ploidy was analyzed with flow cytometry. Results After the tumor cells were transfected with pIRE-S2-EGFP-ESP1 plasmid, the expression of esp1 gene was up-regulated （P 〈 0.01 ）, chromosome number decreased （ P 〈 0.01 ）, and chromosome ploidy reduced （P 〈 0.01 ）. Conclusion The over-expression of esp1 gene inhibits the abnormal proliferation of tumor cells and decreases chromosome ploidy.