EGFR、COX-2及P63蛋白在非小细胞肺癌中的表达及其意义

Expressions of EGFR,COX-2 and P63 in non-small cell lung cancer and their significance

目的检测表皮生长因子受体（epidermal growth factor receptor，EGFR）、环氧合酶-2（cyclooxygenase-2，COX-2）及P63蛋白在人非小细胞肺癌（non—small cell lung cancor，NSCLC）中的表达情况，探讨其与NSCLC临床TNM分期及淋巴结转移的关系。方法采用免疫组化（S-P法）检测EGFR、COX-2及P63蛋白在78例NSCLC中的表达情况。结果EGFR、COX-2及P63蛋白在NSCLC组织中的阳性表达率分别为65．4％（51／78）、61．5％（48／78）和56．4％（44／78）。COX-2和P63的阳性表达在肺鳞癌和腺癌之间具有显著性差异（P〈0．01）。EGFR阳性表达在肺鳞癌和腺癌中无显著差异（P〉0．05）。EGFR和COX-2在NSCLC临床TNM Ⅲ～Ⅳ期和有淋巴结转移组阳性表达率均高于TNM Ⅰ～Ⅱ期和无淋巴结转移组（P〈0．01）。P63蛋白阳性表达率与TNM分期及淋巴结转移无显著差异（P〉0．05）。结论EGFR、COX-2在NSCLC组织中高表达可能促进肺癌的侵袭和转移，COX-2和P63可作为肺鳞癌和腺癌鉴别的参考指标。

Objective To investigate the expressions of epidermal growth factor receptor （EGFR）, cyclooxygenase-2 （COX-2） and P63 protein in non-small cell lung cancer （NSCLC） and their relationship with TNM staging and lymph node metastasis of NSCLC. Methods Seventy-eight paraffin-embedded specimens of NSCLC from 1998 -2005 were collected in this study. Inclusion criteria included no chemotherapy or radiotherapy before operation. Pathological diagnosis was made after operation： 43 squamous carcinoma and 35 adenocarcinoma, 45 with lymph node metastasis and 33 without, 13 in stage Ⅰ, 19 in stage Ⅱ, 28 in stage Ⅲ and 18 in stage Ⅳ. The expressions of EGFR, COX-2 and P63 were determined by immunohistochemical staining （S-P）. Results The expression rates of EGFR, COX-2 and P63 were 65.4% （51/78）, 61.5% （48/78） and 56.4% （44/78） respectively in 78 cases of NSCLC. Significant difference in the expressions of COX-2 and P63 was found between squamous carcinoma and adenocarcinoma （P 〈0.01 ）. The expression of EGFR in NSCLC was not associated with histological types of NSCLC （P 〉 0.05 ）. The positive rate of EGFR and COX-2 protein expressions in NSCLC of stage Ⅲ-Ⅳ and NSCLC with lymph node metastasis was significantly higher than that in stage Ⅰ-Ⅱ and NSCLC without lymph node metastasis （P 〈 0.01 ）. The positive expression of P63 in NSCLC was not associated with TNM staging and lymph node metastasis （P 〉 0.05 ）. Conclusion Over-expressions of EGFR and COX-2 may play an important role in invasion and metastasis of NSCLC. COX-2 and P63 may be valuable markers in differentiating pulmonary squamous cell carcinoma from pulmonary adenoearcinoma.