流动注射纳米微反应器化学发光法测定盐酸二氧丙嗪

Flow-injection Chemiluminescence Analysis of Dioxopromethazine Hydrochloride with Nanometer Microreactor

在酸性条件下,盐酸二氧丙嗪分子中氮原子被质子化后与阴离子AuCl4^-形成离子缔合物，缔合物又被二氯甲烷带入鲁米诺的氯化十六烷基三甲基铵反胶束纳米微反应器中，离解出的AuCl4^-立即与鲁米诺产生化学发光。在一定浓度范围内，发光强度与盐酸二氧丙嗪的含量呈线性关系，可间接测定盐酸二氧丙嗪的含量。在HCl、AuCl4^-、鲁米诺、碳酸钠分别为0．05mol／L、20mg／L、0．4×10^-4mol／L和0．3mol／L（pH=11．5），R（[H2O]／[CTAC]）值为11，泵速均为3．6mL／min（单管），聚四氟乙烯管的内径为0．8mm，进样体积为120uL，e至流通池间的距离为8cm，反应盘管的长度为120mm，直径为2mm，负高压为-650V，二氯甲烷为萃取剂的条件下，线性范围为0．001～20mg／L，检出限（3σ）为0．06μg／L，对质量浓度为1．0mg／L的盐酸二氧丙嗪进行11次平行测定，RSD为1．4％。方法可用于片剂和生物体液中盐酸二氧丙嗪的测定。

Under acidic condition, the nitrogen in dioxopromethazine hydrochloride was easily protonated to form ion-complex with negative ion AuCl4^- , which was extacted and carried by dichloromethane. The ioncomplex entered a reversed micella of cetyhrimethylammonium chlorine containing luminol, and the dissociated AuCl4^- reacted with luminol and produced an analytical chemiluminescnece signal. Under the optimum conditions, i. e., the concentrations of hydrochloric acid, AuCl4^-, luminol and sodium carbonate were 0.05 mol/L, 20 mg/L, 0.4 × 10^-4 mol/L and 0.3 mol/L（pH = 11.5）, respectively, the R（[H2O]/ [ CTAC] ） value was 11, the flow rate（ per tube） was 3.6 mL/min, the inner diameter（ i. d. ） of PTFE tubing was 0. 8 mm, the sampling volume was 120 μL, the distance between e and injection valve was 8 cm, a glass coil（120 mm, 2 mm i. d. ） was used as the flow cell, the photomultiplier tube was operated at -650 V, the extraction solvent was dichloromethane, the linear range was 0. 001 to 20 mg/L and the limit of detection was 0. 06μg/L with a relative standard deviation（n = 11 ） of ca. 1. 4% for 1. 0 mg/L dioxopromethazine hydrochloride. The method has been applied to the determination of the drug in tablets and biological fluids successfully.