检测结直肠组织中人乳头瘤病毒DNA的研究

DELECTION OF HUMAN PAPILLOMAVIRUS DNA IN COLORECTAL CARCINOMA

用人乳头瘤病毒（HPV）高度保守的通用引物和16、18型特异引物对123例给直肠组织作聚合酶链反应（PCR）检测HPVDNA，总检出率为14．60％正常粘膜、结肠炎和炎性息肉组为3．3％（2／71），乳头状腺瘤为17．6％（3／17），原位癌和浸润癌为37．1％（13／35）．在正常组织、癌旁组织和癌组织HPVDNA阳性率分别为2．9％、5．7％和37.1％．在结直肠腺癌中HPV感染以18型多见，18型与16型之比为3:1，HPV在左半结肠以下比右半结肠感染率高。

Piopsy spesmens specimens from colorectum of 123 pahents were analysised for human papiIlomavirus (HPV) by polymerase chain reachon (PCR). We used genera consensus primers and type-specific primers (16,18). HPV DNA was delected in 14.6% of Piopies-There were two of 17 in normal (3.3%), colorechhs and inflammatory polyps, three of 17 in papillate adenomas (17.6%) and thirteen of 35 in colorectal. Detection rate of HPV DNA in normal, adjacent carcinoma and carcinoma were 13 / 35 (37.1%), 2 / 35(5.7%) and 1 / 35(2.9%)respechvely. HPV was predominahy type 18 in colorectal carcinoma. The inflammatory rate is higher in rectum and left colon than right colon.