胰腺癌TFPI-2蛋白表达与细胞凋亡

The effect of TFPI-2 on apoptosis of pancreatic carcinoma histiocyte

目的:探讨胰腺癌组织中细胞凋亡与TFPI-2基因表达的相互关系,研究TFPI-2对胰腺癌细胞凋亡的影响。方法:应用免疫组化技术检测TFPI-2基因在50例胰腺癌及9例正常胰腺组织中的表达,并应用TUNEL法检测细胞凋亡,计算凋亡指数;体外将TFPI-2基因真核表达载体转染人胰腺癌细胞株Panc-1细胞,DNA片段化实验检测细胞凋亡。结果:TFPI-2在胰腺癌中的表达低于正常胰腺组织,TFPI-2指数分别为73.28±9.63和81.42±12.25,P<0.05,差异有统计学意义。TFPI-2的表达及细胞凋亡与临床分期及转移有关,Ⅰ、Ⅱ期及无转移的胰腺癌组织中TFPI-2指数及AI值均高于Ⅲ、Ⅳ期及有转移的胰腺癌组织,TFPI-2阳性组织中的AI值高于TFPI-2阴性组织的AI值,P<0.05,差异有统计学意义;DNA片段化实验证实TFPI-2能诱导Panc-1细胞凋亡。结论:胰腺癌组织中细胞凋亡与TFPI-2的表达水平有关,TFPI-2可能在诱导胰腺癌细胞凋亡中起重要作用。

Objective：To investigate the relation between apoptosis of pancreatic carcinoma histiocyte and expression of TFPI - 2, and evaluate effect of TFPI - 2 on apoptosis of Panc - 1 cells. Methods： The expression of TFPI - 2 was detected by immunohistochemistry and the apoptosis of pancreatic carcinoma histiocyte was detected using TUNEL in 50 pancreatic carcinoma and 9 pancreatic tissues. Then TFPI -2 gene was transfected into Panc - 1 cells. DNA fragment analysis was used to detect apoptosis of transfected cells. Results. The expression of TFPI -2 in pancreatic carcinoma was lower than that of pancreatic tissue, the index of TFPI -2 was 73.28 ± 9.63 and 81.42 ± 12.25 ,P 〈0.05. The expression of TFPI -2 and cell apoptosis were related to the clinical stage and metastasis. The TFPI - 2 index and apoptotic index （AI） of pancreatic carcinoma in stage of Ⅲ, Ⅳand with metastasis was lower than that of pancreatic carcinoma in stage of I , lI and with no metastasis. The apoptotic index in pancreatic carcino- ma expressing TFPI -2 was higher than that in pancreatic carcinoma with no expression of TFPI -2,P 〈 0.05. TFPI -2 could induce apoptosis of the traansfected cells. Conclusion：The apoptosis of pancreatic carcinoma histiocyte is related to the expression of TFPI - 2 in pancreatic carcinoma. TFPI - 2 plays an important role in inducing apoptosis of pancreatic carcinoma histiocyte.