扶正养阴方辅助高强度聚焦超声治疗兔肝VX_2肿瘤的实验研究

The therapeutic effect of Fuzhengyangyin decoction assistant with high intensity focused ultrasound on VX_2 liver cancer in rabbits:an experimental study

目的探讨扶正养阴方联用高强度聚焦超声(HIFU)治疗兔肝VX2肿瘤的治疗效果。方法30只兔肝VX2肿瘤模型随机分为HIFU组(HIFU照射)、联用组(HIFU照射+扶正养阴方)和对照组(不予任何治疗),每组10只,治疗后监测肿瘤体积、丙氨酸氨基转移酶(ALT),脏器转移及组织病理学改变等。结果HIFU组与联用组的肿瘤体积较对照组缩小,差异有统计学意义(P<0.05),但HIFU组与联用组差异无统计学意义。治疗后28d,联用组和HIFU组的ALT低于对照组(P<0.05)且联用组降低更明显(P<0.01)。联用组未发生肺转移,HIFU组有2只发生肺转移,对照组全部发生肺转移。超声和病理检查均提示HIFU治疗后肿瘤组织变性坏死。结论扶正养阴方联用HIFU能有效地抑制兔肝VX2肿瘤的生长,可为HIFU联用中药治疗肝癌的临床研究提供实验依据。

Objective To experiment the therapeutic effect of Fuzhengyangyin decoction assistant with high intensity focus ultrasound（HIFU）on the VX2 liver cancer in rabbits.Methods Totally 30 rabbits which induced with VX2 cancer in liver were divided into 3 groups, HIFU group （HIFU therapy）, combination group（HIFU assistant with Fuzhengyangyin decoction）, and control group（without any treatment）, each group involved 10 rabbits. The volume of tumor, ALT, metastasis, and pathological changes were followed up during the stage of treatment. Results The volume of tumor in the combination group and HIFU group decreased obviously than the control group（ P 〈 0.05）, although there was no significant statistical difference between these two treatment groups. Four weeks later, the ALT of HIFU group and the combination group lower than the control group（ P 〈 0.05）, and the combination group got more significant results （ P 〈 0.01 ）. There were no metastasis in combination group, but 2 of HIFU group and all control group were metastasized to lung. Ultrasound and pathological report supported the final results. Conclusion The Fuzhengyangyin decoction assistant with HIFU had better therapeutic effect in VX2 liver cancer in rabbit liver. This primarily experimental study provides the foundation results to the clinic research of the combination therapy on liver tulnor.