金丝慈竹愈伤组织培养及植株再生研究

A Primary Study on Callus Induction and Plant Regeneration of Bambusa affinis' viridiflavus'

通过正交试验筛选出金丝慈竹愈伤诱导的最佳配方MS＋2，4-D5mg／L＋6-BA（0．2～0．5）mg／L；记录了愈伤发生过程中不同类型愈伤的发生时间及生长状态，并通过对健康的Ⅰ型愈伤组织进行增殖培养筛选出最佳增殖配方MS＋2，4-D（2—3mg／L）＋6-BA0、2mg／L＋NAA0．5mg／L。通过对增殖后健康的Ⅰ型愈伤组织的诱导分化，实现了器官再生，并获得最佳芽体分化配方MS＋6-BA5mg／L＋NAA0．2mg／L及不定根系发生诱导配方MS＋6-BA1mg／L＋NAA（0．5～2mg／L）。

The callus induction culture and plant regeneration of Bambusa affinis＇ viridiflavus＇ were investigated in this paper. The results showed that the optimum medium for callus induction culture were MS ＋ 2,4-D 5 mg/L ＋6-BA （0. 2 ～0. 5） mg/L. The different types of callus were observed during the different culture stages, among which the type Ⅰ callus could be further induced with the optimum medium of MS＋2,4-D （2 -3 mg/L） ＋6-BA 0. 2 mg/L ＋ NAAO. 5 mg/L for callus multiplication. According to the experiment, it was found that the better medium of bud differentiation was MS ＋ 6- BA 5 mg/L＋NAA 0.2 mg/L, and the optimum media of adventitious root was MS＋6-BA 1mg/L＋ NAA （0. 5 ～2） mg/L.