摘要
为优化加杨叶总黄酮的提取工艺,通过单因素和正交试验进行优选,所得结果采用方差分析,并进行精密度和回收率试验。结果表明:影响加杨叶总黄酮提取工艺的主要因素为提取温度,其次是液料比和乙醇浓度,而提取时间的影响不大。确定加杨叶总黄酮最佳提取工艺为:时间60 min,乙醇浓度50%,液料比40∶1,温度80℃。此法重现性好,精密度高,平均回收率达98.92%。
The single factor test and the orthogonal experimental design were carried out to optimize the extraction technology of total flavonoids from leaves of Populus canadensis Moench in this paper. The results indicated that the extraction temperature, the ratio of solvent to material and the ethanol concentration had a remarkable influence on the extraction of total flavonolds, while the extraction time had hardly influence on it. The optimal extraction technology was 50% ethanol and the ratio of solvent to material 40：1 at the temperature of 80℃ for 60 min. The optimized technology had good reproducibility and more precision, and the average recovery, was 98, 92%.
出处
《林业科技开发》
2008年第2期48-51,共4页
China Forestry Science and Technology
基金
北京林业大学研究生自选课题基金资助项目(06jj127)
关键词
加杨叶
总黄酮
提取工艺
精密度
回收率
Leaves of Populus canadensis Moench,
Total flavonoids
Extraction technology
Precision
Recovery
