多发性硬化患者血清TNF-α、IFN-γ、IL-10检测及临床意义

Determination of Serum TNF-α,IFN-γ,and IL-10 in Patients with Multiple Sclerosis and Their Clinical Significance

目的探讨肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-10(IL-10)在多发性硬化(MS)免疫病理机制中的作用。方法采用ELISA法对42例MS患者(其中发作期25例,缓解期17例)和25名健康人(对照组)血清中TNF-α、IFN-γ、IL-10进行测定,并进行各组间分析。结果MS患者发作期TNF-α、IFN-γ水平比缓解期及对照组明显升高,IL-10水平比缓解期及对照组明显下降。结论TNF-α、IFN-γ可能与MS免疫病理过程有关,可作为评价MS活动性的辅助指标之一。IL-10对MS病情的缓解及稳定起重要作用,可作为MS缓解的指标。

Objective To investigate the pathogenic role of TNF-α IFN-γ, and IL-10 in multiple sclerosis. Methods Serum TNF-α, IFN-γ, and IL-10 were determined by ELISA in 42 patients with multiple sclerosis and 25 healthy subject. Among 42 patient, 25 were in acute attack stage and 17 in alleviative stage. Results The levels of TNF-α and IFN-γ in cases with acute attack stage of multiple sclerosis were higher than those with alleviative stage and in controls. The levels of IL-10 in cases with acute attack stage of multiple sclerosis were lower than those with alleviative stage and in controls. Conclusion Serum TNF-α and IFN-γ participate in immunopathologic process of MS, may be an useful auxiliary laboratory markers for estimating the status of multiple sclerosis. IL-10 may play an important role in alleviative stage and may be laboratory markers of alleviative stage MS.