低氧习服过程对模拟高原急性低氧大鼠肺组织结构的影响

Effects of hypoxic preconditioning on pulmonary structure of rats exposed rapidly to simulated high altitude hypoxia

目的:观察低氧习服对模拟高原低氧大鼠肺组织的影响。方法:32只Wistar大鼠随机分成4组:常氧对照组(N组,n=8)、急性低氧组(H组,n=8)、3000m低氧习服+急性低氧组(C3组,n=8)、5000m低氧习服+急性低氧组(C5组,n=8)。习服完成后,置于模拟海拔6000m急性低氧24h,观察动物在不同低氧习服水平对模拟高原急性低氧大鼠肺组织结构改变。结果:急性低氧组大鼠肺组织显微和超微结构出现少量的肺泡性肺水肿,明显的间质性肺水肿;而经过低氧习服后,间质性肺水肿明显减轻,基本无肺泡性肺水肿出现。结论:低氧习服可以明显减轻急性低氧大鼠肺水肿特别是间质肺水肿的程度。

Objective：To investigate the effects of hypoxic preconditioning on pulmonary structure of rats exposed rapidly to simulated high altitude hypoxia. Methods：Thirty- two Wistar rats were randomly divided into four groups： normal control group （N group, n= 8）, acute hypoxic group （H group, n=8）, 3 000 m hypoxic preconditioning＋ acute hypoxic group （C3 group, n=8）, 5 000 m hypoxic preconditioning ＋acute hypoxic group （C5 group, n= 8）. After treated with hypoxic preconditioning according to different hypoxic conditions, animals were exposed to a simulated altitude of 6 000 m for 24h Then the structural changes of their lungs were observed. Results：Obvious pulmonary interstitial edema and slight alveolar edema was observed in H group under light microscope and electron microscope. Compared with H group, pulmonary interstitial edema was significantly relieved and less alveolar edema was found in two hypoxic preconditioning groups （C3 group and C5 group）. Conclusions. Hypoxic preconditioning can improve significantly the hypoxic pulmonary edema induced by acute hypoxia in rats.