反相高效液相色谱法同时测定苯妥英钠、卡马西平的血药浓度

Determination of Phenytoin and Carbamazepine Concentration in Serum with RP-HPLC

目的建立反相高效液相色谱法同时测定苯妥英钠、卡马西平的血药浓度。方法采用C18柱(200mm×4.6mm,5μm),甲醇-水(48∶52)为流动相,紫外检测波长为205nm,流速1.1mL/min,柱温40℃。结果两种药物分离良好,苯妥英钠、卡马西平质量浓度线性范围分别为2.14～42.8μg/mL和2.04～20.40μg/mL,方法回收率分别为99.33%和99.64%,提取回收率分别为97.13%和96.64%,日内、日间RSD均小于4%。结论该方法操作简便、快速、准确,可用于临床治疗药物的监测。

Objective To establish a rapid analytical method for the determination of phenytoin （DPH） and carbamazepine （CBZ） in serum by RP-HPLC. Methods The analytical column was C18 column （200 mm ×4. 6 mm,5μm）. The mobile phase was methanol- water （48：52）. The detection wavelength was 205 nm. The flow rate was 1.1 mL/min and the temperature of column was set at 40℃. Results Two drugs were separated well under the chromatographic condition. The linear range of DPH and CBZ were 2. 14-42.8μg/mL and 2.04- 20.40μg/mL respectively. The method recovery rates were 99.33% and 99.64% respectively. The extraction recovery rates were 97. 13% and 96. 64% respectively. The RSD with inter- day and intra - day were both less than 4%. Conclusion This method is accurate, rapid, sensitive and suitable for clinical monitor of DPH and CBZ in serum.