脑胶质瘤和正常脑组织中MGMT、LRP和TopoⅡα的表达及其意义

Expressions of LRP,MGMT and TopoⅡα in Brain Glioma and Normal Brain Tissue and Their Significances

【目的】探讨脑胶质瘤和正常脑组织中三种耐药酶O6-甲基鸟嘌呤-DNA-甲基转移酶(MGMT)、肺癌耐药相关蛋白(LRP)、拓扑异构酶Ⅱα(TopoⅡα)的表达及其意义。【方法】利用两步法免疫组织化学技术检测MGMT、LRP、TopoⅡα三种耐药酶在脑胶质瘤和正常脑组织的表达。【结果】三种耐药酶在正常脑组织中几乎不表达;在脑胶质瘤中表达强度与患者的性别、年龄和肿瘤的大小无关(P>0.01),但与肿瘤的WHO分级明显相关(P<0.01)。WHO分级越高,表达越强。【结论】胶质瘤的发生与三种耐药酶的表达改变有关;联合测定多种耐药酶的表达对判定肿瘤的恶性程度与制定化疗方案有参考价值;纠正耐药蛋白的表达有可能成为胶质瘤基因治疗的新策略。

[Objective]To investigate the expressions of lung resistance-related protein （LRP）, O^6-methylguanine-DNA methyltransferase （MGMT） and rioctyl-phosphine oxide Ⅱα （Topo Ⅱα） in brain glioma and normal brain tissue as well as to elucidate their significances. [MethodslThe expressions of the three drug-resistance enzymes were detected by immunohistochemical techniques. [Results]The expressions of the three drugresistance enzymes in normal brain tissue were almost all negative. The expressive intensities of them in brain glioma were not related to patients＇ sex, age and size of neoplasms （ P 〉0.05）, but were significantly associated with the WHO classification of glioma（ P 〈0.01）. The poorer was the differentiation of brain glioma, the stonger would be the expressions of LRP, MGMT and Topo Ⅱα. [Conclusion]These data suggest that the occurrence of glioma is correlated with the expressions of the three drug-resistance enzymes. It is significant to detect theirs expressions simultaneously when judging the malignant degree of glioma and selecting chemotherapeutic plan for an individual patient. Future research in the field of drug-resistance enzymes will provide new strategies for the gene therapy of glioma.