摘要
介绍了水溶性共轭高分子和核酸适体(aptamer)在生物传感检测中的应用。提出了一种基于aptamer和水溶性聚噻吩的构象效应进行凝血酶检测的比色方法。结果表明,该方法具有较高的选择性和灵敏度,肉眼目测最低可检测63nM凝血酶。
We introduce the application of water-soluble conjugated polymers and aptamer in the detection of biomolecules. Then a colorimetric protocol based on aptamer and conformational effects of water-soluble polythiophene is reported. It is demonstrated that the protein sensor we designed is highly selective and sensitive, and the lowest detection limit with naked eyes is 63 nM.
出处
《南京邮电大学学报（自然科学版）》
EI
2008年第1期59-63,共5页
Journal of Nanjing University of Posts and Telecommunications：Natural Science Edition
基金
国家自然科学基金(0404016)
南京邮电大学引进人才科研启动基金(NY207037)资助项目
