合并前列腺炎的良性前列腺增生组织中Ki-67,Bcl-2,Bax和caspase-3表达及意义

Expression of Ki-67,Bcl-2,Bax and caspase-3 in benign prostatic hyperplasia combined with prostatitis and their significances

目的:通过检测单纯良性前列腺增生(benign prostatic hyperplasia,BPH)与合并前列腺炎的BPH患者中Ki-67,Bcl-2,Bax和caspase-3蛋白表达差异,探讨炎症与BPH发生发展的关系。方法:收集58例行经膀胱前列腺摘除手术的BPH患者的前列腺标本,常规HE染色后观察其合并前列腺炎情况,其中单纯BPH16例,合并前列腺炎的BPH42例。采用免疫组化和Western免疫印迹技术研究Ki-67,Bcl-2,Bax和caspase-3在前列腺组织中的表达情况。结果:合并前列腺炎的BPH患者Ki-67和Bcl-2表达有显著升高(P<0.05),caspase-3表达显著下调(P<0.05),但Bax的表达无明显变化(P>0.05)。结论:前列腺炎促进BPH细胞Ki-67和Bcl-2蛋白表达,减少caspase-3蛋白激活,提示炎症可能参与了BPH的发生发展过程。

Objective To detect the expression of Ki-67, Bcl-2, Bax and caspase-3 in simple benign prostatic hyperplasia （BPH） and BPH combined with prostatitis, and to evaluate the effect of inflammation on the development and progression of BPH. Methods All specimens were obtained from patients undergoing surgical resection of the prostate. The paraffin section of the specimens was stained with hemotoxyline and eosin, and observed under light microscope to examine the inflammation hispathological changes. Sixteen patients with simple BPH （Group A ） and 42 patients with BPH combined with prostatitis （Group B ） were included. Immunohistochemical analysis and Western blot were used to examine the expression of Ki-67, Bcl-2, Bax and caspase-3. Results The expression of Ki-67 and Bcl-2 was significantly higher in Group B than that in Group A （ P 〈 0.05 ） , and caspase-3 expression was significantly lower （P〈0.05 ） . There was no difference in Bax expression between the 2 groups （P 〉 0.05 ）. Conclusion Prostatitis can up-regulate Ki-67, Bcl-2 expression, and down-regulate the expression of caspase-3 in BPH. Prostatitis appeared to play an important role in the development of BPH by affecting the proliferation and apoptosis of the prostatic cells.