污水人工快速渗滤系统中厌氧氨氧化菌的分子生态学分析

Molecular ecology of anammox bacteria in constructed rapid infiltration system

使用厌氧氨氧化菌的引物从污水人工快速渗滤系统(CRI)砂层样品总DNA中扩增16S rDNA序列,并测序、构建系统发育树,从而确定了其发育地位。结果表明:在CRI系统6号样品(50cm深)中存在厌氧氨氧化菌,测定该菌的16S rDNA部分序列(长度为831bp)与anoxic biofilm clone Pla1-48、uncultured anoxic sludge bacterium KU2和anaerobic ammonium-oxidizing planctomycete KOLL2a等厌氧氨氧化菌的相似性均在97%以上,表明它们的系统发育关系比较相似。厌氧氨氧化菌的发现为此工艺的改进和反硝化能力的提高提供了新的思路。

In this paper, the specific-16S rDNA primers of anammox bacteria from constructed rapid infiltration system （CRI） were used for PCR amplification, cloning, and sequencing, and the phylogenetic tree was constructed. The anammox bacteria were found at the depth of 50 cm in CRI, arid the 16S rDNA partial sequence was 831 bp. Phylogenetic analysis indicated that the anammox bacteria were closed to anoxic biofilm clone Plal-48, uncultured anoxic sludge bacterium KU2, and anaerobic ammonium-oxidizing planctomycete KOLL2a, with the similarity of DNA sequence being above 97%. The finding of anammox bacteria in CRI provided a new approach for the technical improvement and the increase of denitrification in CRI.