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Pichia pastoris表达系统提高表达量的几个关键点 被引量：1

Some Key Points of Improvement of Pichia pastoris Expression System
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摘要 Pichia pastoris是近年来迅速发展的一种真核生物表达宿主,有着广阔的应用前景.本文从提高P.pastoris表达重组蛋白产量的角度,就启动子、高拷贝重组子、密码子偏爱、宿主菌和高密度发酵等方面的优化进行了综述. Pichia pastoris has been proved to be a promising host organism for the production of heterogonous proteins. We report here the optimization of promoter, high copy integrants, codon preference, host genotype, and high-density cell culture of Pichia pastoris to increase the production of hetcrogonous proteins and make it more efficient for large-scale production.
作者 周细南 张爱联 张添元 罗进贤 谭燕华
机构地区 中国热带农业科学院热带生物技术研究所 中山大学生命科学学院
出处 《海南大学学报（自然科学版）》 CAS 2008年第1期68-73,共6页 Natural Science Journal of Hainan University
基金 海南省重点科技计划项目(05204)
关键词 PICHIA PASTORIS 表达系统 产量 Pichia pastoris Expression system Output
分类号 Q786 [生物学—分子生物学]
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参考文献53

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二级参考文献19

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海南大学学报（自然科学版）
2008年 第1期

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