利用RT-PCR快速检测水稻条纹叶枯病病毒

Detecting Rice Stripe Virus(RSV) in Infected Rice Plants by RT-PCR

根据水稻条纹叶枯病病毒基因组序列的信息,在其RNA聚合酶基因、外壳蛋白基因和RNA沉默抑制子基因以及特异蛋白基因的两侧设计4对引物,对感染病毒的水稻植株总RNA并进行RT-PCR扩增,可以在感病植株中分别扩增出水稻条纹叶枯病病毒基因组特有的4个条带,并通过测序和Genbank blast证实。利用该方法可以对水稻和其它寄主进行条纹叶枯病病毒早期检测。

Rice stripe disease, caused by rice stripe virus（RSV）, has caused great yield loss in Jiangsu province in recent years. The identification of RSV in the small brown planthopper（Laodelphax stri- atellus）, rice and host plant is of great importance for the disease forecasting. Four pairs of primers to amplify RNA dependent RNA polymerase and the coat protein（CP）and disease-specific protein （SP） and putative RNA silencing suppressor （NS3） were designed. Four bands with about 170 bp to 300 bp were obtained in infected rice plants, respectively. The separate segment was legated to pGEM-T-easy vector, then sequenced to conform. This approach can be used for early detection of RSV in infected rice and other host plants.