氦氖激光照射对兔正畸牙周组织中血管内皮细胞生长因子受体-2表达的影响

Effects of He-Ne Laser Irradiation on Vascular Endothelial Cell Growth Factor R-2 Expression in Periodontium of Tooth Movement in Rabbits

为深入研究氦氖激光照射对促进实验性牙移动牙周组织血管改建的作用机制,应用波长632.8 nm,功率20 mW的氦氖激光照射实验性牙移动兔牙周组织,通过免疫组化染色与图像分析观察分析实验结果。结果表明弱激光照射侧血管内皮细胞生长因子(VEGF)受体-2的表达,无论在压力区还是张力区均较对照侧出现得早,峰值高,持续的时间长。停止照射后,照射侧血管内皮细胞生长因子受体-2的表达水平仍高于对照侧,提示停止弱激光照射后这种生物刺激作用仍能持续。表明弱激光照射能有效促进血管内皮细胞生长因子受体-2的表达,促进正畸牙周组织的血管化与骨改建。

To study the mechanism of helium neon laser promoting blood vessel rebuilds in periodontium of rabbits experimental tooth movement in rabbits, He-Ne laser with the wavelength of 632.8 nm and power of 20 mW was used to irradiate periodontium of experimental tooth movement of rabbits. The result was analysised by immunohistochemistry dyeing and image analysis. The experiment show that the vascular endothelial cell growth factor （VEGF） R-2 expression where the low level laser irradiation, regardless of comparison in the pressure zone or the tension area, appears earlier, have higher peak power and longer time than it does in side irradiating. After stopping the irradiation, the expression level of the irradiation side of the VEGFR-2 is still higher than the comparison side. It shows that the biologic effect still continue after stopping the low level laser irradiation. The result indicates that the low level laser irradiation can promote the VEGFR-2 expression effectively, thus promotes the vascularization of orthodontic dental capsule and the rebuilding of bone.