ATMFS对犬骨盆弓状线部力学性能和胶原合成的影响

Effects of acetabular tridimensional memory fixation system on the mechanical property and collagen synthesis of canine iliopectineal crest

目的:探讨骨盆髋臼三维记忆内固定系统(acetabular tridimensional memory fixation system,ATMFS)对犬骨盆弓状线部力学性能和胶原合成的影响。方法:选用10只成年杂种家犬,双侧髋臼臼顶上方1.5 cm处横形截骨,分别采用ATMFS前柱固定器和6孔重建钢板内固定,于术后2、4、6、8、12周各取2只动物,行X线检查、大体观察、生物力学测试及Masson三色法染色组织学观察。结果:ATMFS侧骨折端无凌乱骨痂,骨折愈合时间明显快于钢板侧,胶原纤维的排列较钢板侧规则有序,与骨皮质的方向一致。术后第4周开始,ATMFS侧骨折端生物力学强度明显高于钢板侧(P<0.05),两组骨折端新骨生成面积之间存在显著性差异有(P<0.05)。结论:ATMFS的解剖位固定和其产生的持续顺应生理力线的压应力能够刺激骨折端胶原结构的重建及力学性能的恢复,促进骨折早期愈合。

Objective：To explore the effects of acetabular tridimensional memory fixation system （ATMFS） on the mechanical property and collagen synthesis of canine iliopectineal crest. Methods： 10 adult mixed breed dogs without significant individual difference were made transverse fracture at bilateral iliopectineal crest 1.5 cm above the dome of acetabulum,which were fixed with ATMFS and steel plate respectively. X-ray, macropathological observation, and biomechanical test were performed at 2,4,6,8, and 12 weeks after operation. Samples from the fracture gaps were investigated by Masson staining and image pattern analysis of new bone formation. Results：There was no disordered bony callus at ATMFS sides and healing time of ATMFS sides was obviously faster than that of steel plate sides. The mechanical property of ATMFS sides was superior to that of steel plate sides from 4 week after operation （P〈0.05）. There was significant difference of the integrated optical density （IOD）of new bone formation between two sides at 4,6,8 and 12 weeks （P〈0.05）. Conclusions： Anatomic fixation and persistent compression corresponding with physiological line of force provided by ATMFS can promote bone healing by stimulating reconstruction of bone collagen and recovery of mechanical property.