压应变作用下组织工程软骨的构建

The Effect of Compressive Strain on Tissue Engineered Cartilage Construction in vitro

在压应变作用下构建组织工程化软骨。以大鼠的骨髓间充质干细胞(BMSCs)为种子细胞,以壳聚糖海绵为支架材料,利用压应变加载装置对细胞-壳聚糖海绵支架材料的复合物进行体外动态加载,7d后植入大鼠背部皮下,免疫组织化学染色检测Ⅱ型胶原蛋白的分泌,甲苯胺蓝染色检测酸性糖胺多糖的分泌;利用材料试验机对组织块进行生物力学性能检测,从弹性模量方面对比工程化软骨与正常软骨的差异。在大小为1%、频率为1Hz的压应变条件下,构建的组织工程化软骨经体内移植后,分泌软骨基质Ⅱ型胶原及酸性糖胺多糖,其弹性模量较植入前显著增强。在压应变作用下,细胞复合壳聚糖支架材料可以形成初级的组织工程化软骨。

To investigate the effect of compressive strain on tissue engineered cartilage construction in vitro. Take rat marrow mesenchymal stem cells （BMSCs） as seeding cells and chitosan sponge as scaffolds. Cell-scaffold constructs were applied a dynamic compression by a compressive strain device for 7days, and the composites were implanted in rat 7 days later. Immunohistochemical and Tofuidine blue experiments were performed for type Ⅱ collagen and GAG secretion respectively. And then, correlative estimation on biomechanics were conducted to investigate the difference of elastic modulus between tissue engineered cartilage and normal cartilage. Tissue engineered cartilage produced under 1% and 1Hz compressive strain loading condition excreted cartilage matrix, and its elastic modulus increased obviously. Due to the compressive strain, primary tissue engineered cartilage was formed from the cell- chitosan scaffold construct.