肾移植术后急性排斥受者外周血中CD95定量表达水平的动态观察

The dynamic expression of CD95 in renal transplantation recipients with acute rejection

目的监测肾移植术后急性排斥受者外周血中T淋巴细胞表面CD95定量表达的动态变化。方法采集肾移植受者不同时间段的抗凝静脉血与相应荧光标记的单克隆抗体反应,用双色流式细胞术定量分析T淋巴细胞表面CD95的表达量,同时分析健康对照组CD95的定量表达。结果健康成人T淋巴细胞表面CD05的表达量为(6 226.5±2 162.3)分子/细胞,无性别及年龄差异(P>0.05);肾移植术后稳定组受者CD95的表达量为(7 323.4±1 769.8)分子/细胞,与健康对照组比较差异无统计学意义(P>0.05)。肾移植受者在术后出现急性排斥时CD95表达水平明显升高[(23 107.4±10 517.7)分子/细胞],免疫冲击治疗后呈下降趋势,至治疗2个月后CD95的表达水平与稳定组差异已无统计学意义(P>0.05)。结论CD95参与了肾移植术后急性排斥反应的免疫过程,其表达水平可作为肾移植术后受者急性排斥反应的辅助诊断指标,且在评估治疗效果中具有重要意义。

Objective To analyze the dynamic expression of CD95 on CD3^＋ cells in renal transplantation recipients with acute rejection （AR）. Methods In 10 renal transplantation reeipients,antieoagulated venous blood samples from different stage were collected and mixed with corresponding fluorescence labeled monoclonal antibodies. CD95 expression on CD3^＋ cells was quantitatively measured by dual color flow eytometry, simultaneously CD95 expression from normal healthy volunteers were analysed. Results The expression level of CD95 on CD3^＋ cells in heahhv volunteers was （6 226.5±2 162.3） molecules per cell and no difference in age and sex（P 〉 0.05 ）. The difference between heahhv volunteers and stable recipients （ [ 7 323.4 ±1 769.8 ] molecules per cell ） was not significant （ P 〉 0.05 ）. CD95 expression on CD3^＋ cells was significantly increased in renal transplanted recipients with All （[ 23 107.4 ±10 517.7 ] molecules per cell） compared to that in renal stable recipients without rejection. There was a descendent trend after therapy. The difference between stable recipients and All was not significant in 2 months after therapy（P 〉 0.05）. Conclusions CD95 on CD3^＋ eells nlay modify the immunological situation of the recipients after renal transplantation. CD95 level can be regarded as a reference marker for renal transplantation recipients with AR, and can be useful to assess the treatment.