MMP-9、TIMP-1及VEGF在宫颈癌中的表达及相关性研究

Research on Expression and Correlativity Among MMP-9,TIMP-1 and VEGF in Cervical Carcinoma

目的研究基质金属蛋白酶9(MMP-9)、组织金属蛋白酶抑制剂1(TIMP-1)及血管内皮生长因子(VEGF)在宫颈癌组织中的表达与临床病理和预后的关系。方法采用免疫组化SP法检测35例宫颈癌、31例宫颈上皮内瘤样病变(CIN)和12例正常宫颈组织中MMP-9、TIMP-1及VEGF的表达情况,探讨三者的表达与临床病理和预后的关系。结果宫颈癌中MMP-9、TIMP-1和VEGF的阳性率较正常宫颈组与CIN组均增高。MMP-9与VEGF的阳性表达与组织分化高低和是否有淋巴结转移有显著相关性,与临床分期、病理类型之间无显著相关性,而TIMP-1的阳性率仅与是否有淋巴结转移有相关性。结论MMP-9和VEGF表达增强而TIMP-1的相对增强不明显可能使宫颈癌更易发生侵袭转移。

Objective To investigate the correlativity with expression and clinical pathological mechanism and prognosis of matrix metalloprotein-9 （ MMP-9 ）, tissue inhibitor of metalloproteinase-1 （ TIMP-1 ） and vascular endothelial growth factor （VEGF） in cervical carcinoma. Methods The expression of MMP-9, TIMP-1 and VEGF by SP immunohistochemistry method in 35 cervical carcinoma tissues, 31 cervical intraepithelial neoplasms（CIN） and 12 normal cervical tissues were detected to discuss the relationship of expression and clinical pathological mechanism and prognosis between the triple. Results The positive rates of MMP-9, TIMP-land VEGF in cervical carcinoma tissues increased more obviously than those in normal cervical tissues and CIN groups. Positive expression of MMP-9 and VEGF had notable correlativity with tissue differentiation and the metastasis of lymph node, but the relation with clinical periodization or pathological mechanism type was not notable. The positive rates of TIMP-1 had correlativity only whether having lymph node metastasis or not. Conclusion The expression on strengthening of MMP-9 and VEGF whereas relative but no obvious strengthening of TIMP-1 possibly makes cervical carcinoma easier to cause invasive metastasis.