基质金属蛋白酶-9在涎腺黏液表皮样癌中的表达

Expression of Matrixmetalloproteinases-9 in Salivary Mucoepidermoid Carcinoma

目的探讨基质金属蛋白酶-9(MMP-9)的表达与涎腺黏液表皮样癌细胞增殖活性和转移的关系。方法采用免疫组织化学SP法检测42例涎腺黏液表皮样癌标本中MMP-9及PCNA的表达。结果MMP-9在涎腺黏液表皮样癌中的表达与淋巴结转移、TNM分期密切相关(P<0.05),而与患者的年龄、性别、肿瘤分化程度无关(P>0.05)。有淋巴结转移、TNMⅢ+Ⅳ期者,MMP-9的表达分别明显高于无淋巴结转移、TNMⅠ+Ⅱ期者(P<0.05)。黏液表皮样癌中MMP-9与PCNA的表达呈正相关(r=0.621,P<0.01)。结论MMP-9在黏液表皮样癌细胞的增殖和转移中起重要作用,可作为判断涎腺黏液表皮样癌恶性潜能的生物学指标。

Objectie To investigate the expression of matrixmetalloproteinase-9 （MMP-9） and its correlation with cancer cell proliferation and metastasis in salivary mucoepidermoid carcinoma （MEC）. Methods Immunohistochemical SP method was used to detect the expression of MMP-9 and PCNA in 42 cases of MEC. Results The expression of MMP-9 was closely correlated with lymph nodes metastasis and TNM stage of MEC （P 〈0. 05）. There was no significant correlation between MMP-9 expression and gender, age or tumor differentiation （P 〉 0. 05 ）. In cases with lymph nodes metastasis and TNM stage Ⅲ ＋ Ⅳ, the expression of MMP-9 was significantly higher than that with no lymph nodes metastasis and TNM stage Ⅰ ＋ Ⅱ （P 〈 0. 05 ）. A positive correlation was found between the expression of MMP-9 and PCNA in MEC （r = 0. 621 ,P 〈 0. 01 ）. Conclusion The expression of MMP-9 may play an important role in cancer cell proliferation and metastasis of MEC, and can be considered as a biological indicator of malignant potential of MEC.