我国不同生态类型桑树地方品种遗传多样性的ISSR分析

Genetic Diversity of Mulberry Local Varieties From Different Ecotype as Revealed by ISSR Analysis in China

利用ISSR分子标记评价了我国不同生态类型的66个桑树地方品种的遗传多样性。12个ISSR引物总扩增条带数为83条，其中50条为多态性条带，多态性比例为60．24％，平均PIC值为0．1469；平均遗传相似系数为0．8456，8个生态群体间的遗传相似系数变异范围为0．8441～0．9640，不同地方品种间的遗传多样性存在差异。根据ISSR标记遗传相似系数，按UPGMA法对66个地方桑树品种进行聚类分析，聚类结果与生态型有一定相关性。研究结果提示：保护不同生态类型的桑树群体，对丰富桑树种质资源具有重要意义。

Evaluation of genetic relationship is important for long term improvement in mulberry yield, quality and resistance, and for germplasm conservation and identification. In this study 66 mulberry local varieties from different ecotypes in China were analyzed by ISSR markers. Twelve SSR primers generated a total of 83 amplification products, of which 50 were polymorphic, revealing 60.24% polymorphism among 66 mulberry local varieties, and the mean PIC value was 0. 146 9, The mean genetic similarity coefficient was 0, 845 6, indicating that genetic diversity of different local varieties existed variation, A dendrogram of all 66 local varieties of mulberry based on the genetic similarity using ISSR markers was generated by UPGMA cluster method. In the dendrogram, most varieties from the same ecotype clustered together. The above results showed that the conservation of mulberry populations from different ecotypes would be useful for germplasm resources.