桑树内生真菌的分离、鉴定及其多样性分析

Isolation and Identification of Endophytic Fungi in Mulberry and its Diversity Analysis

用组织分离法从不同桑树品种的器官组织中共分离出内生真菌114株,经显微形态观察,鉴定114株内生真菌分别归属于镰孢霉属Fusarium、链格孢属Alternaria、柱霉属Scytalidium等21个属,表明桑树内生真菌具有丰富的生物多样性。不同桑树品种树体内的内生真菌分布有一定差异。同一桑树品种不同器官组织的内生真菌种类和数量差异较大,根中最多,茎中次之,叶中最少。其中:镰孢霉属为根中优势种群,链格孢属为茎、叶中的优势种群;丛梗孢属Monilia和镰孢霉属菌株在根、茎、叶中均能分离到,其它属的菌株只存在于其中的某一个或两个器官组织。研究结果有助于进一步探讨桑树内生真菌与生理活性物质的关系。

One hundred and fourteen strains of endophytic fungi were isolated from the roots, stems and leaves of different mulberry varieties using the tissue culture method. Through the morphological identification, they belonged to 21 genera, including Fusarium, Altemaria, Scytalidium etc. The result showed endophytic fungi in mulberry exhibited the broad biodiversity. The distribution of endophyte was different among mulberry varieties. The type and quantity of endophytic fungi had great difference among different organs of the same variety, roots 〉 stems 〉 leaves. Fusarium was the dominant population in roots, while Alternaria was the dominant population in stems and leaves. Monilia and Fusarium were isolated from three organs, and the others were isolated from one or two organs.