一株桑树内生拮抗细菌的分离鉴定及生物学特性研究

Isolation and Identification of an Antagonistic Endophytic Bacterium From Mulberry and its Biological Charcteristics

从健康桑树叶片中分离到一株内生拮抗细菌L144,该菌株对多种植物病原真菌及病原细菌均有较强的抑制作用。通过形态学观察、生理生化指标测定、16S rDNA碱基序列测定和同源性分析,鉴定该菌株为枯草芽孢杆菌,定名为Bacillus subtilisL144。该菌株已在GenBank注册,登录号为EU118756。对菌株部分生物学特性研究表明,其生长的最适pH值为6.5,最适生长温度为33℃,能广泛利用碳源,氮源。

L144 strain, isolated from the healthy mulberry leaves, was an antagonistic bacterium that exhibited excellent resistant against various plant pathogens. The strain was identified as Bacillus subtilis based on the result of its 16S rRNA gene sequence homology, and the physiological and biochemical characteristics. The sequence of 16S rDNA obtained in this study had been registered at GenBank database （Accession number EUl18756）. The determination results of biological characteristics showed that L144 strain could use many substances as the sources of carbon and nitrogen, and the optimal pH and temperature for the growth were 6. 5 and 33 ℃, respectively.