应用于家蚕F2代分离群体的两种连锁作图方法的作图效果比较

Analysis on F_2 Population of Bombyx mori Using Two Different Mapping Methods

构建精细的家蚕分子连锁图谱需要合适的作图方法。应用Mapmaker/EXP3.0和F2lnkgsilk两种作图方法,对家蚕F2代91个个体为分离群体的300个AFLP标记和69个个体为分离群体的470个RAPD标记数据进行了比较分析。用F2lnkgsilk作图方法分析得到的分群数与Mapmaker/EXP3.0作图方法相比有所增加,而总的图距却显著增加,平均图距也有较大的增加,但产生的二联体数基本相同。就单个连锁群而言,两种作图分析方法的连锁标记及其数目80%以上相同,但是标记间的排列顺序70%以上有差异;用F2lnkgsilk作图方法分析得到的单个连锁群的总图距和平均图距也相应增大。

A molecular linkage map with high quality and high density is the base to construct a detailed map for all the major genes influencing quantitative traits. A suitable mapping software is especially needed. Here a segregating population had been studied,which consisted of 91 F2 individuals with 300 AFLP markers and another 69 F2 individuals with 470 RAPD markers. The resulting data were analyzed using two different mapping softwares, Mapmaker/EXP3.0 and F21nkgsilk The amount of segregating groups from F21nkgsilk were slightly increased and the numbers of two-marker-group were similar. However, the total genetic distance derived from F21nkgsilk was significantly longer with moderate increase in average genetic distance compared with Mapmaker/EXP3.0. As to single linkage group, when the results were analyzed with the two softwares mentioned above, there were 80% similarities in linkage markers and their numbers used, although more than 70% these markers were different in arrangement order. The total genetic distance and its average one of single linkage group derived from F21nkgsilk was increased correspondingly.