显微分光光度计对水体有机污染物诱导的转化细胞DNA含量的分析

Analysis on DNA content of trasformation cells induced by extracts of water using microspectrophotometer

在水源水及自来水有机提取物诱导人胚肺（ＢＫＭ）细胞恶性转化基础上，运用ＭＰＶ－ＳＰ型显微分光光度计对Ⅲ型转化灶中细胞的ＤＮＡ含量进行了分析。结果表明：水样在实验剂量范围内可不同程度地诱导细胞转化灶的形成，并有明显的剂量反应关系。与源水相比，自来水有较强的潜在致癌性，从Ⅲ型转化灶克隆中挑取的细胞ＤＮＡ含量增高，与阴性对照比有显著性差异，说明转化细胞形态学改变与细胞ＤＮＡ含量的改变有一定的关系。

Based on the study of the potential carcinogenicity of source water and drinking water of a certain city by BKM cells maligmant transformation, the DNA content of focic derived cells were analysised using microspectrophotometer.The results showed that extracts of both tap water and drinking water could induce BKM cell malignant transformation. Comparing with negative cells, a higher absorbance value representing DNA content of transformative cells was observed.There is a certain relationship between the cell morphological change and the DNA content.