应用^(32)P-后标记技术对苯醌和氢醌处理人血淋巴细胞形成DNA加合物的研究

Detection of DNA adducts in human blood lymphocytes treated with hydroquinone and p benzoquinone by 32 P-postlabeling

应用核酸酶Ｐ１促进的３２Ｐ－后标记技术对苯醌和氢醌处理人血淋巴细胞后形成的ＤＮＡ加合物进行了研究。结果表明，人血淋巴细胞被苯醌和氢醌处理２４ｈ之后，均形成了同一种ＤＮＡ加合物。两种化合物在不同浓度下处理人血淋巴细胞每１０７核苷酸中有０．０１～１０个核苷酸形成加合物。为了达到同等ＤＮＡ加合物水平，氢醌比苯醌需要更高的浓度。小牛胸腺ＤＮＡ与苯醌、氢醌反应可生成５种ＤＮＡ加合物。用苯醌或氢醌处理淋巴细胞所形成的加合物种类与上述纯ＤＮＡ与苯醌，氢醌形成的５种加合物在双向薄层层折图谱上完全不相符合。这些结果意味着苯醌、氢醌与ＤＮＡ的加合作用在细胞内存在着修饰机制。

In this paper,DNA adducts in human blood lymphocytes treated with hydroquinone (HQ) or p benzoquinone (BQ) were detected by the Nuclease PI enhanced 32 P-postlabeling technique,It was shown that the treatment of lymphocytes with HQ or BQ produced same DNA adducts.The DNA adduct level varied from 0.01 to 10 adducts per 10 7 nucleotides as a function of the treatment concentration for both chemicals.To obtain the same DNA adduct level required higher concentrations of HQ than BQ.Reaction of calf thymus DNA with HQ or BQ produced five adducts.The DNA adducts formed in lymphocytes treated with HQ or BQ did not correspond to any of the adducts formed in reaction of calf thymus DNA with HQ or BQ.This result suggests that there is a cellular mechanism to modify DNA adduct formation by HQ and BQ.