HPLC法测定芪丑汤中大黄素的含量

Determination of Emodin in Qichoutang by HPLC

目的:建立中药复方芪丑汤中大黄素的含量测定方法。方法:采用 HPLC 法,色谱柱为 DIKMA ODS C_(18)(4.6mm×200ram,5μm),流动相甲醇-0.1%磷酸溶液(90:10),检测波长:440nm。结果:大黄素在0.22～1.1μg之间与峰面积呈良好线性关系,r=0.9999;大黄素的平均回收率为98.3%,RSD=1.76%(n=6)。结论:该方法分离效果好,操作简单、准确,可作为芪丑汤的质量评价。

Objective： To determine the content of Emodin in Qichoutang.Methods：A HPLC method was established.The chromatcgraphic column was DIKMA ODS C18 Columns （4.6mm×200mm,5μm）.The mobile phase was methanol -0.1% phosphoric acid solution （90：10） and the detection wavelength was at 440nm.Results：The standard curve was linear in the range of 0.22-1.1μg.The correlation coefficient was 0.9999.The average recovery rate for Emodin was 98.3% and RSD was 1.76%（n=6）.Conclusion：The method is effective,simple and accurate and it provides scientiffic indexes for quality control of Qichoutang.