HPLC β-环糊精手性流动相添加剂的研究　Ⅲ.β-环糊精手性选择性包合稳定常数的简化计算及验证

An Investigation of β Cyclodextrin Chiral Mobile Phase Additive in HPLC Ⅲ The Simplified Calculation and Experimental Verification of the Stability Constants of Enantioselective Inclusion Complexation with β Cyclodextrin

用简化的计算公式对不同的色谱条件下的β－环糊精流动相手性添加剂对甲基苯巴比妥对映异构体的保留行为的影响进行了的探讨，同时探讨了β－环糊精包合稳定常数与流动相中溶剂强度的关系。结果表明简化的数学模型具有运算简便、误差小和线性范围宽的特点。

Based on the literature and experimental results, investigation on the effect of β cyclodextrin as mobile phase additive on the retention behaviour of methylphenobarbital enantiomers was carried out with a simplified equation for the calculation of inclusion complex constant The relationship between the inclusion complex constants of β cyclodextrin and solvent strenth was also studied The results indicated that the simplified mathematical model had the advantages of simpler calculation, smaller error and better linearity