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老年大鼠脑内多巴胺转运体的表达 被引量：1

The expression of dopamine transporter in aged rat brains
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摘要 目的观察老年大鼠脑内多巴胺转运体(DAT)的表达变化。方法健康雄性Wistar大鼠3月龄、12月龄和24月龄组各20只;应用免疫组织化学和免疫印迹技术观察大鼠脑内多巴胺转运体在衰老过程中的表达。结果大鼠黑质、尾壳核多巴胺转运体免疫反应强度在24月龄组明显低于3月龄(P<0.01)及12月龄组(P<0.05);24月龄组大鼠黑质、尾壳核DAT与β-actin免疫印迹条带相对吸光度的比值低于3月龄和12月龄组(P<0.05,P<0.01)。结论大鼠脑内多巴胺转运体的表达随衰老而减少。 Objective To study the altered expression of dopamine transporter （DAT） in aged rat brains. Methods The healthy male Wistar rats were divided into 3-, 12- and 24-month-old groups （n = 20）. Immunohistochemistry and Western-blot were used to detect the altered expression of DAT protein in aged rat brains. Results DAT immunoreactive intensity in substantia nigra and caudate putamen in 24-month-old group was lower than that in 3-month-old （P 〈0. 01 ） and 12-month-old （P 〈0. 05） groups. The ratio of DAT/13-actin in substantia nigra or caudate putamen of 24-month-old rats was significantly lower than that in 3- and 12-month-old rats （ P 〈 0. 05, P 〈 0. 01 ）. Conclusions The expression of DAT in aged rat brains decreases with aging.
作者 康云霄 王磊 王智涛 岂春晓 李双成 石葛明
机构地区 河北医科大学神经生物研究室
出处 《中国老年学杂志》 CAS CSCD 北大核心 2008年第6期527-529,共3页 Chinese Journal of Gerontology
基金 河北省教育厅计划攻关项目资助(项目编号2007129) 河北省科学技术研究与发展指导课题(项目编号062761385)
关键词 多巴胺转运体 酪氨酸羟化酶 大鼠 Dopamine transporter （DAT） Tyrosine hydrexylase Rat
分类号 R322.81 [医药卫生—人体解剖和组织胚胎学]
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参考文献10

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同被引文献11

  • 1Spencer TJ, Biederman J, Madras BK,et al. ^123I-FP-CIT semi-quantitative SPECT detects preclinical bilateral dopaminergic deficit in early Parkinson's disease with unilateral symptoms[J]. Nucl Med Commun,2005 ;26 (5) :421-6.
  • 2Spencer TJ, Biederman J, Madras BK, et al. Further evidence of dopamine transporter dysregulation in ADHD:a controlled PET imaging study using altropane[J]. Biol Psychiatry ,2007 ;62 ( 9 ) : 1059-61.
  • 3Spencer TJ, Bicderman J, Madras BK, et al. In vivo neuroreceptor imaging in attention-deficit/hyperactivity disorder:a focus on the dopamine transporter[ J]. Biol Psychiatry ,2005 ;57( 11 ) : 1293-300.
  • 4Erixon-Lindroth N, Farde L, Wahlin TB ,et al. The role of the striatal dopamine transporter in cognitive aging [J]. Psychiatry Res, 2005 ; 138 (1) :1-12.
  • 5Tupala E,Hall H,Bergstrom K,et al. Different effect of age on dopamine transporters in the dorsal and ventral striatum of controls and alcoholics [ J]. Synapse,2003 ;48(4) :205-11.
  • 6Van Dyck CH, Seibyl JP, Malison RT,et al. Age-related decline in dopamine transporters: analysis of striatal subregions, nonhnear effects, and hemispheric asymmetries [ J). Am J Geriatr Psychiatry, 2002 ; 10 ( 1 ) : 36 - 43.
  • 7Bannon MJ ,Whitty CJ. Age-related and regional differences in dopamine transporter mRNA expression in human midbrain [J]. Neurology, 1997 ; 48 (4) :969-77.
  • 8Collier TJ, Lipton J, Daley BF, et al. Aging-related changes in the nigrostriatal dopamine system and the response to MPTP in nonhuman primates:diminished compensatory mechanisms as a prelude to parkinsonism [J]. Neurobiol Dis,2007;26(1) :56-65.
  • 9Melchitzky DS, Erickson SL, Lewis DA. Dopamine innervation of the monkey mediodorsal thalamus : Location of projection neurons and ultrastructural characteristics of axon terminals[ J ]. Neuroscience,2006 ; 143 (4) :1021-30.
  • 10Wilson JM, Levey AI, Rajput A, et al. Differential changes in neurochemical markers of striatal dopamine nerve terminals in idiopathic Parkinson's disease[J]. Neurology, 1996 ;47 ( 3 ) :718-26.

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中国老年学杂志
2008年 第6期

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