印迹基因PEG10在胃癌中的表达及其与幽门螺杆菌感染的关系

Correlation of PEG10 expression and its relation to helicobacter pylori infection in human gastric carcinoma

目的探讨印迹基因PEG10在胃癌组织中的表达特点及其与幽门螺杆菌(Hp)感染的关系。方法采用逆转录聚合酶链反应(RT-PCR)检测40例胃癌、癌旁组织中印迹基因PEG10的mRNA水平;采用免疫组织化学染色技术及Warthin-Starry银染法检测42例胃癌、癌旁组织及6例正常胃组织中PEG10蛋白的表达及Hp感染情况。结果RT-PCR结果显示20例胃癌组织中9例(45.0%)PEG10mRNA表达,对应的癌旁组织仅有2例(10%)表达;免疫组织化学染色结果显示42例胃癌组织中22例(52.38%)PEG10蛋白表达阳性,对应的癌旁组织仅有5例(11.90%)PEG10蛋白表达阳性,正常胃组织无PEG10蛋白表达;Hp银染法结果显示42例胃癌组织中25例(59.52%)为Hp感染阳性,癌旁组织20例(47.62%)Hp感染阳性;22例PEG10阳性表达胃癌组织中20例Hp感染阳性。结论PT-PCR结果与免疫组织化学染色结果具有一致性,即PEG10mRNA及蛋白在胃癌组织和癌旁组织中的表达率比较差异有显著性(P<0.05);PEG10在胃癌组织中的表达与Hp感染呈正相关(P<0.05)。

Objective To investigate the expression of a.novel imprinted gene, PEG10, in human gastric carcinoma, and its ralationship to helicobacter pylori （Hp） infection. Methods PEG10 mRNA expression in 40 cases of gastric carcinoma and the corresponding adjacent normal tissues were detected by RT-PCR. PEG10 protein expression in 84 cases of gastric carcinoma, and the corresponding adjacent normal tissues and 6 cases of normal gastric tissues were detected by immunohistochemistry. The infection of Hp was measured by Warthin.Starry stain. Results RT-PCR result showed PEG10 mRNA expression in gastric carcinoma of 9 cases （45.00%） and in correspond- ing adjacent normal tissues of 2 cases （ 10. 00% ）. Immunohistochemistry results showed positive PEG10 protein expression in gastric carcinoma of 22 cases （52. 38% ） and in corresponding adjacent normal tissues of 5 cases （ 11.90% ）, no expression of PEG10 in nomal gastric tissues. Warthin-Starry stain showed among 42 cases of gastric carcinoma, positve Hp infection in gastric carcinoma tissue of 25 cases （59. 52% ）, in corresponding adjacent normal tissues of 20 cases （47. 62% ） ; there were 20 cases of positive Hp infection in gastric carcinoma among 22 cases of PEG10 positive expression. Conclusions RT-PCR result is same as immunohistochemistry stairming＇s, there is significant difference in PEG10 mRNA and protein between gastric tissue and the corresponding adjacent normal tissue. PEG10 overexpressed in gastric carcinoma is positively correlated with Hp infection.