大鼠断层供皮区创面愈合过程中TGF-β_1基因表达的研究

TGF β 1 GENE EXPRESSION IN THE HEALING PROCESS OF SKIN WOUND IN RAT

为了观察创面愈合过程中转化生长因子－β１（ＴＧＦ－β１）的基因表达，以大鼠断层供皮区创面为模型，应用原位杂交、斑点杂交等方法对内源性ＴＧＦ－β１ｍＲＮＡ在创面愈合过程中的表达变化进行了观察。结果表明，ＴＧＦ－β１ｍＲＮＡ主要在成纤维细胞、上皮细胞及毛细血管内皮细胞中表达，其表达量在伤后第６天达峰值，以后随上皮化的完成，ＴＧＦ－β１ｍＲＮＡ表达水平逐渐降至正常。提示，在创面愈合过程中ＴＧＦ－β１基因有明显表达，其表达规律与创面愈合时间有密切关系。认为。

For observation of the change of transforming growth factor β 1 (TGF β 1) gene expression in the process of skin wound healing, the following experiments were performed. Sixteen Wistar rats were chosen. At each side of the rats back, a 1 cm×1.5 cm middle thick skin wound was made. After 3, 6, 9 and 12 days, the specimens were taken from the wounds. For each specimen, half of it was used for RNA extraction, and underwent dot blotting; and the other half was frozen immediately and underwent in situ hybridization. The probes were dig labeled PDGF BB cDNA probe and TGF β 1 probe. The results showed that TGF β 1 gene was expressed mainly in fibroblast, epithelial cell and capillary endothelial cell. The peak of TGF β 1 mRNA content was in the 6th day postoperatively. After that, the content of TGF β 1 decreased to normal. It was suggested that TGF β 1 gene expression was in close relation with healing process. TGF β 1 may play an important regulatory role in the skin wound healing.