RT-PCR方法检测病毒性心肌炎病原体

DETECTION OF THE PATHOGEN IN VIRAL MYOCARDITIS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION

应用逆转录聚合酶链反应（ＲＴ－ＰＣＲ），对１０４０例疑似病毒性心肌炎患者的静脉血进行检测，共检出各种病毒感染者６１２例（５８．８４％）。其中检出感染柯萨奇病毒３８０例（６２．０９％），埃可病毒１２９例（２１．０７％），柯萨奇与埃可病毒合并感染８２例（１３．３０％），脊髓灰质炎病毒２１例（３．４３％）。结果表明，柯萨奇病毒的检出高于其它种病毒（Ｐ＜０．０５）。心电图有异常改变的占２０％，主要是ＳＴ段下降及房室传导阻滞等最多见。用ＲＴ－ＰＣＲ技术诊断病毒性心肌炎病原体，只需要４小时，方法简便、灵敏度高，可为临床诊断病毒性心肌炎提供有力佐证。

To detect pathogen of viral myocarditis,venous blood from 1 040 suspected patients was tested by reverse transcriptase polymerase chain reaction(RT-PCR).612 cases had pathogens including 380 patients ( 62.09% ) with Coxackievirus,129 patients ( 21.07% ) with Echovirus, 82 patients ( 13.3% )with concurrent Coxsackievirus and Echovirus and 21 patients ( 3.43% ) with Poliomyelitis virus . The result shawed the detection of Coxackievirus was higher than others.Electrocardiogram abnormalities mainly S-T depression and auriculoventricular block occurred in 20% of the patients. RT-PCR assay took only 4 hours.The technique is easy to perform and highly sensitive,which provides a strong evidence for diagnosing viral myocarditis.