相同栽培条件下4种甘草根中总黄酮含量的比较

Comparson of the total flavonoids content in roots of four cultivated licorice under the same growing condition

目的:比较研究相同栽培条件下乌拉尔甘草、胀果甘草、光果甘草、刺果甘草根中总黄酮的含量。方法:采用冷浸乙醇提取法对4种6年生栽培甘草根中的总黄酮进行了提取分离,用硝酸铝显色法测定和比较分析总黄酮的含量。结果:相同栽培条件下乌拉尔甘草、光果甘草、胀果甘草、刺果甘草根中总黄酮含量分别为0.41%,0.31%,0.17%,0.05%。结论:以相同栽培条件下4种6年生甘草根中总黄酮的含量为指标评价其质量优劣,栽培乌拉尔甘草质量最好,其次是光果甘草和胀果甘草,栽培刺果甘草不宜作为黄酮类药物的药源植物。

OBJECTIVE To comparing the contents of total flavonoids in roots of euhivated Glycyrrhiza uralensis,Glycyrrhiza glabra,Glycyrrhiza in flata and Glycyrrhiza pallidiflora under the same growing condition. METHODS The total flavonoids in roots of the four cultivated licorice were extracted and separated by ethanol at room temperature. The contents of the total flavonoids in the extractives were analyzed by ultraviolet visible speetrophotometry with Al（NO3 ）3 coloration method. RESULTS The contents of total flavonoids in roots of cultivated Glycyrrlhiza uralensis ,Glycyrrhiza glabra,Glycyrrhiza inflata and Glycyrrhiza pallidiflora were 0. 41%, 0. 31%,0.17%and 0.05% respectively. CONCLUSION The qualily of Glycyrrhiza uralensis is the best one in the four cultivated licorice according to the contents of total flavonoids in roots and cultivated Glycyrrhiza pallidiflora can＇t be used as flavonoids resource plant.