重组抗原在戊型肝炎病毒感染诊断中的应用

Serodiagnosis of hepatitis E virus infection by recombinant antigen based enzyme immunoassay

目的探讨重组抗原在戊型肝炎病毒（ＨＥＶ）感染诊断中的应用。方法用ＨＥＶ重组抗原建立酶免疫试验（ＥＩＡ）检测肝病患者和健康人群血清中抗戊型肝炎病毒抗体（抗－ＨＥＶ）。结果５５份用新加坡ＤＢＬ公司抗－ＨＥＶ诊断试剂盒检测为抗－ＨＥＶ阳性的肝炎患者血清中，５３份（９６．４％）用本方法检测也为阳性；４５份ＤＢＬ试剂盒检测为抗－ＨＥＶ阴性的血清中，本法检测有３份为抗－ＨＥＶ阳性。检测甲、乙、丙型肝炎患者血清各３０份，抗－ＨＥＶ阳性率分别为１３．３％（４／３０）、１３．３％（４／３０）和１０．０％（３／３０）。健康人抗－ＨＥＶ阳性率为５．０％（８／１６０）。重组抗原与合成肽抗原混合用于ＥＩＡ，可以检出两种抗原单独阳性的抗－ＨＥＶ。结论以重组抗原为基础的ＥＩＡ特异性强、灵敏度高、快速简便。

Objective To diagnose HEV infection an enzyme immunoassay (EIA) based on recombinant antigen encoded by open reading frame 2 (ORF 2) of HEV was used. Methods The antibody to HEV in sera of patients with hepatitis and healthy people was detected by EIA. Results Of 55 anti HEV positive sera by the kit from Diagnostic Biotechnology Ltd, (DBL) Singapore, 53 were positive for anti HEV and in 3 of 45 the negative sera were positive by the EIA. In the patients with hepatitis A, B and C viruses, anti HEV positive rates were 13.3% (4/30), 13.3%(4/30) and 10.0%(3/30) respectively. Eight of 160 healthy persons were positive for anti HEV. Conclusions The recombinant antigen based EIA will be helpful in the diagnosis and epidemic investigation of hepatitis E.