脾胃湿热证模型大鼠胃黏膜AQP_3、AQP_4基因的表达

Expression of Aquaporin3,4 Gene in Gastric Mucosa of Rat Model with Pi-Wei Damp Heat Syndrome

目的探讨脾胃湿热证大鼠动物模型建立方法及脾胃湿热证模型大鼠胃黏膜水通道蛋白AQP3、AQP4基因表达。方法40只大鼠随机分为四组,造模20d,对照组正常喂饲;脾虚组隔日喂饲,非喂饲日灌喂番泻叶水煎液;模型组20%蜂蜜水自由饮用,隔日灌服油脂,每日2%水杨酸钠灌胃。造模开始后第16～20日每日20:00至次日8:00将大鼠置入人工气候箱中;治疗组前20d处理同模型组,第21～25日灌服清热化湿方。观察结束后,FQ-PCR方法测定大鼠胃黏膜AQP3、AQP4基因表达。结果模型大鼠在造模过程中出现脾胃湿热证的特征性表现;模型组AQP3、AQP4基因表达水平高于对照组、脾虚组、治疗组。结论通过对大鼠施加内、外湿因素的造模方法可复制出临床脾胃湿热证的证候特征;AQP3、AQP4的异常表达可能是脾胃湿热证的发生机制之一。

Objective： To establish the animal model of Pi - Wei damp heat syndrom （PWDHS）and to explore the expression of aquaporin3,4（AQP3,4） gene in gastric mucosa of PWDHS, Methods： forty SD rats were randomly devided into 4 groups and observed 20 days. Group A（normal group, NG） was fed with routine method, Group B（Pi deficiency syndrome group, PDSG）was fed alternatively with routine method and aqua of cassia angnstifolia Vahl. Group C（PWDHS） was fed with fat, honey liquid, 2% sodium salicylate（q, o. d） and treated with damp heat environment. Group D（Treatment group, TG）was fed and treated sinlilar to group C and fed with Qingre Huashi Recipe from 21 to 25 day, The expression of AQP3.4 gene in the 4 groups were determined by fluorescence quantitative polymerase chain reaction （FQ -PCR）. Results： The syndromes and signs in Group C were simiar to the clinical Pi -Wei damp heat Syndrome; Expression of AQP3, AQP4 gene in PWDHS was statistical significance higher than that in PDSG, TG and the NG, Conclusion： The method of feeding fat, honey and sodium salicylate under damp heat environment is effective to copy PWDHS. Abnormal expression of AQP3.4 gene maybe one of the PWDHS pathogenesis.