蛇床子总香豆素对骨质疏松大鼠骨密度、骨形态计量学影响

Effect of Cnidiummonnier(L) Cusson on Bone Mineral Density and Trabecular Area in Experimental Osteoporosis Rats

目的探讨蛇床子总香豆素对骨质疏松大鼠骨密度、骨形态计量学影响以及与血钙、磷、维生素D代谢和生长因子的关系。方法制备骨质疏松大鼠模型(OVX)。实验动物分为模型对照组(OVX组,摘除卵巢、不作大鼠处理)、蛇床子总香豆素治疗组(治疗组,摘除卵巢后用蛇床子总香豆素治疗)、盐酸雷洛昔芬治疗组(对照组,摘除卵巢后用雷洛昔芬治疗)、假手术组(Sham组,仅切除卵巢周围的脂肪组织)。检测大鼠腰椎、股骨上段骨密度值(BMD)、股骨骨小梁面积、矿化沉积率、血清IGF-1和25-OH维生素D以及血淋巴细胞维生素D受体(VDR)含量。结果治疗组、对照组均较OVX组腰椎、股骨上段骨密度增高,治疗组较对照组股骨上段骨密度增高。治疗组骨小梁面积明显增加、矿化沉积率增高。治疗组较对照组血清IGF-1浓度值、血清25-OH维生素D浓度值升高,与OVX组比较差异显著。各组血淋巴细胞VDR含量无明显变化,与OVX组相近(P>0.05)。结论蛇床子总香豆素能够预防腰椎、股骨上段骨密度丢失,使骨小梁面积明显增加,矿化沉积率增高,且血清IGF-1及血清25-OH维生素D浓度值升高,但对VDR含量无明显影响。

Objective： To observe the effect of Cnidiummonnier（L） Cusson on osteoporosis of ovariectomized rats. And investigate the association between Cnidiummonnier（L） Cusson and IGF- 1 and vitamin D. Methods： 40 female 10 month old SD rats were divided into 4 groups： sham group, OVX group, treatment group and control group. The experimental model of osteoporosis was established by removing twin ovary operation. Given Cnidiummonnier（L） Cusson 12mg/kg body weight P. O. for 8 weeks, bone mineral densities（BMD） of global and the lumber spine and femur neck were measured with hologic dual energy X -ray bone densitometer in rat. Femur trabecular area, bone mineral depostion were measured after the end of therapy. Serum IGF - 1 levels and serum 25 - OH VitD concentrations and lymphocyte Vitamin D receptor contents were determined by means of ELISA. Results： BMD of the lumber spine and femur neck in treatment group and control group were higher than that in OVX group. Cnidiummonnier（L） increased trabecular area, bone deposition compared with OVX group. The serum IGF - 1 and 25 - OH VitD concentration in treatment group and control group were significantly higher than that in OVX group. Conclusion： Using Cnidiummonnier（L） Cusson has effective therapy for osteoporosis and demonstrated definite alterations in IGF - 1 levels and Vitamin D metabolite.