中国汉族人HLA-DQAl~*0601基因转录启动子区显示新的核苷酸序列

A New W Box Sequence of HLA-DQA1~*0601 Gene Promoter in a Chinese Homozygous Cell SMY-43A

本文测定了中国汉族人ＨＬＡ纯合细胞ＳＭＹ－４３Ａ的ＤＱＡ１＾＊０６０１基因启动子区（ＱＡＰ）核苷酸序列，发现顺式作用元件Ｗ ｂｏｘ中－２１５和－２１６位核苷酸组成与已报道的白种人ＤＱＡ１＾＊０６０１的ＤＡＰ序列不同，由于ＳＭＹ－４３Ａ的Ⅱ类基因单倍型组成为中国人特有，提示相应的ＱＡＰ多态性可能具有人种差异。

For highly polymorphic HLA class D genes, transcription promoter re-gions are essential in determining the differential patterns of allelic expression in re-lation to their functions. A 318 base pair fragment of QAP (DQAl promoter) of a Chinese HLA-DQAl*0601 homozygous cell SMY-43A, including cis-acting element X, Y,Y2 and W box, was PCR-amplified and sequenced. Our results showed sequence difference of the two nucleotides in site -215 and -216 of W box (as G and C) when compared with the nucleotides of correspondent DQAl*0601 QAP (as A and G) in Caucacian, which implys that the QAP polymorphism of SMY-43A might be unique for Chinese population.