虾青素对成骨细胞羟自由基氧化损伤的影响

Effects of Astaxanthin on the damage of osteoblast induced by H_2O_2

目的:在H2O2胁迫下,研究虾青素增强成骨细胞抗自由基损伤的作用。方法:用H2O2作用MC3T3E1成骨细胞,建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、虾青素低剂量组(1×10-7mol/L)、虾青素中剂量组(1×10-6mol/L)和虾青素高剂量组(1×10-5mol/L)。测定不同处理组细胞活力、超氧化物歧化酶(superoxi dedismu-tase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量,同时利用荧光偏振法测定细胞膜流动性。结果:模型组细胞活力、SOD活性、细胞膜流动性均比各剂量虾青素组显著降低(P﹤0.01),而ROS、LPO含量均比各剂量虾青素组显著升高(P﹤0.01)。结论:H2O2摄入会导致大鼠成骨细胞的氧化损伤,而虾青素可以预防或降低此类损伤对细胞的影响。

Objective ：To investigate the effect of Astaxanthin on enhancing the function of anti-oxidative damage in osteohlast. Methods：MC3T3-E1 osteohlasts were randomly divided into five groups,including control group,model group, Astaxanthin group [low-dose （ 1 ×10^-7 mol/L）, middle-dose （ 1 ×10^-6 mol/L）, high-dose （ 1 × 10^-5 mol/L） ], in which the activity of cells, activity of superoxide dismutase （SOD）,the content of reactive oxygen species （ROS）,lipid oxygen （LPO）and membrane fluidity were tested and compared. Results：Compared with Astaxanthin groups ,the activity of cells ,SOD activity and membrane fluidity in the model group were significantly decreased（P 〈 0.01 ）. However,the contents of ROS and LPO were significantly raised （P 〈 0.01 ）. Conclusion ： H2O2 can cause oxidative damage of MC3T3-E 1 osteohlasts, hut Astaxanthin can prevent or decrease its influence.