烧伤大鼠肺组织一氧化氮水平的改变

Effects of thermal injury on production of nitric oxide in rat lung

目的：研究一氧化氮（ＮＯ）在烧伤后肺损伤中的作用。方法：选用ＳＤ大鼠，麻醉后制成２０％Ⅲ度烧伤。分成对照组、烧伤组和烧伤＋单甲基精氨酸（Ｌ－ＮＭＭＡ）组。伤后定期取肺组织测定ＮＯ－２／ＮＯ－３和ｃＧＭＰ，并分析ＮＯ与肺含水量之间的关系。结果：烧伤后肺组织ＮＯ和ｃＧＭＰ水平均明显升高，肺含水量也相应增加。给予一氧化氮合成酶特异性抑制剂Ｌ－ＮＭＭＡ后，随着肺内ＮＯ的减少，肺含水量也显著降低。结论：烧伤后所造成的肺损伤可能与肺内一氧化氮合成酶升高，产生大量ＮＯ有关。

Objective:To elucidate the relationship between thermal injury and production of lung nitric oxide (NO).Methods:The levels of lung NO - 2 /NO - 3( the stable end products of NO ) and cGMP in rats with full thickness burn of 20% TBSA were measured in 3, 6, 12 and 12 h after burn injury. Results: A higher level of lung NO - 2 /NO - 3 was observed from 3 through 24 h postburn. Parallel to the changes of NO - 2 /NO - 3,lung tissue cGMP level and lung water content were increased in burned rats.L NMMA,a specific inhibitor of NO synthase, could block the rises of lung NO - 2 /NO - 3 and lung water content. Conclusion:The lung injury caused by burns may be associated with induction of nitric oxide synthase and formation of NO.