纽荷尔脐橙CRTISO基因片段的克隆与分析

Cloning and Analysis of CRTISO Gene Fragment from Newhall Navel Orange

利用RT-PCR技术，从纽荷尔脐橙（Citrus sinensis Osbeck）果实cDNA中分离出类胡萝卜素异构酶（Carotenoid isomerase，CRTISO）基因片段。该片断长446bp，编码148个氨基酸。用BLASF比较其氨基酸同源性发现，该片段氨基酸残基与伏令夏橙（C．sinensis Osbeck）、番茄（Lycopersicon esculentum Miller）和拟南芥（Arabidopsis thaliana）CRTISO的同源性分别为99％、85％和83％，与里斯本柠檬（C．limon Burm．F．）和温州蜜柑（C．unshiu Marc．）完全一致。半定量RT-PCR分析表明，CRTISO基因在8～11月份纽荷尔果实中均有表达，10月份的表达量最大。

The cDNA sequence for carotenoid isomerase （CRTISO） has been cloned from fruits of Newhall navel orange by RT- PCR technique. Sequencing results revealed that it shared the length for 446bp, encoding 148 amino acids. BLAST results showed that the deduced amino acid shared 100% identities with those of Lisbon limon and Satsuma Mandarin, 99% identities with Valencia sweet orange CRTISO, 85% and 83 % identities with those of Lycopersicon esculentum Miller and Arabidopsis thaliana, respectively. Semi-quantitative RT- PCR results showed that expressions of CRTISO gene in fruits of Newhall navel orange were detected from August to November, with the highest one in October.