HBV／HEV二价疫苗性抗原基因在E．coli中表达条件优化的初步研究

Preliminary study on optimization of expression condition of vector expressing HBV/HEV bivalent vaccinal antigen in E.coli

为有效提高由乙型肝炎的S抗原基因和戊型肝炎ORF2编码区羧基末端414—606氨基酸基因片段构建的高效表达载体质粒PTΩ-T7SE在大肠杆菌BL21(DE3)中的表达效率,研究了质粒稳定性、葡萄糖质量分数、pH值、IPTG浓度、转接量、诱导时间、诱导温度、溶解氧对目的蛋白相对产量的影响,优化得到了较好的培养方法,使目的蛋白的相对含量达48.23%,菌体干重达0.5963g/L.结果表明,采用优化方法可制备较高纯度蛋白.本研究为大规模的发酵工艺提供了依据.

In order to improve the production of interest protein expressed by plasmid PTΩ-T7SE, which contains both encoding genes. The S antigen of type B hepatitis and the 414-606 amino acide of C-teminal of E hepatitis are associated with protective immunity responses. Studied the effection of recombinant plasmid stability, glucose concentration, pH, IPTG concentrat, inoculum size, induction time, induction temperature, and weel as dissolved oxygen concentration during the expression of interest proteins. By the optimized method,the relative amount of interest proteins is 48.23% and the dry weight of thallus is 0. 596 3 g/L. Thus , this study is useful for large-scale production